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Nanoscale Imaging: Methods and Protocols (Methods in Molecular Biology)

This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules including high-speed imaging and probing with AFM; probing chromatin structure with magnetic tweezers; and fluorescence correlation spectroscopy on genomic DNA in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and through, Nanoscale Imaging: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing and expanding field.

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Preface... Table of Contents... Contributing Authors... Part I Imaging and Probing of Biomolecules 1. High Resolution Atomic Force Microscopy Imaging of Nucleic Acids Pablo Ares, Julio Gomez-Herrero, and Fernando Moreno-Herrero 2. Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing Rong Zhu, Hermann J. Gruber, and Peter Hinterdorfer 3. AFM-Based Single-Molecule Force-Spectroscopy of Proteins Zackary N. Scholl and Piotr E. Marszalek 4. High Resolution AFM-Based Force Spectroscopy Krishna P. Sigdel, Anna E. Pittman, Tina R. Matin, and Gavin M. King 5. Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions Sibaprasad Maity, Ekaterina Viazovkina, Alexander Gall, and Yuri L. Lyubchenko 6. Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezers Franziska Kriegel, Willem Vanderlinden, Thomas Nicolaus, Angelika Kardinal, and Jan Lipfert 7. AFM-Based Characterization of Electrical Properties of Materials John Alexander, Sergey Belikov, and Sergei Magonov 8. Supported Lipid Bilayers for Atomic Force Microscopy Studies Zhengjian Lv, Siddhartha Banerjee, Karen Zagorski, and Yuri L. Lyubchenko 9. Quantifying Small Molecule Binding Interactions with DNA Nanostructures Xuye Lang, Yingning Gao, and Ian Wheeldon Part II High-Speed AFM of Biomolecules 10. Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy Takayuki Uchihashi, Hiroki Watanabe, and Noriyuki Kodera 11. High Resolution and High-Speed Atomic Force Microscope Imaging Francesca Zuttion, Lorena Redondo-Morata, Arin Marchesi, and Ignacio Casuso 12. High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies Takahiro Watanabe-Nakayama and Kenjiro Ono 13. Direct Observation of Dynamic Movement of DNA Molecules in DNA Origami Imaged using High-Speed AFM Masayuki Endo and Hiroshi Sugiyama 14. Assembly of Centromere Chromatin for Characterization By High-Speed Time-Lapse Atomic Force Microscopy Micah P. Stumme-Diers, Siddhartha Banerjee, Zhiqiang Sun, and Yuri L. Lyubchenko 15. High-Speed Force Spectroscopy for Single Protein Unfolding Fidan Sumbul, Arin Marchesi, Hirohide Takahashi, Simon Scheuring, and Felix Rico Part III Imaging and Probing of Biomolecular Complexes 16. Probing RNA-Protein Interactions with Single-Molecule Pull-Down Assays Mohamed Fareh and Chirlmin Joo 17. Preparing Frozen-Hydrated Protein-Nucleic Acid Assemblies for High-Resolution Cryo-EM Imaging Panchali Goswami, Julia Locke, and Alessandro Costa 18. Probing Chromatin Structure with Magnetic Tweezers Artur Kaczmarczyk, Thomas B. Brouwer, Chi Pham, Nynke H. Dekker, and John van Noort 19. Single Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly Jaya Sarkar and Sua Myong 20. Correlative Atomic Force and Single Molecule Fluorescence Microscopy of Nucleoprotein Complexes Herlinde De Keersmaecker, Wout Freder

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Nanoscale Imaging: Methods and Protocols (Methods in Molecular Biology)

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