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Protein Engineering by Semisynthesis

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<p>As genomics gives way to proteomics as the focus of scientific imagination in the biological sciences, more emphasis will be placed on the technology and interpretation of protein engineering experiments. Protein engineers will become increasingly sophisticated in the questions that they pose and demanding of the tools available to change protein structure. The optimal way to introduce non-coded amino acids for mechanistic studies, or site-specific reporter atoms for spectroscopic structural biology, is by protein semisynthesis. In Protein Engineering by Semisynthesis, the leading practitioners of the method cover their individual protein of expertise forming a comprehensive illustration of the various methods developed. By covering the most recent philosophical and methodological approaches and developments of semisynthesis and peptide synthesis to date, this book provides further understanding of the principles of protein structure-function relationships gained from semisynthetic analog in addition to providing a comprehensive and comprehensible laboratory guide. This book focuses on recent developments which synergistically combine chemical and molecular biological techniques that have made semisynthetic manipulations much easier to undertake. TOC:Protein Semisynthesis.- Methodological Advances in the Last Decade.- Stereoselective and Chemoselective Religation.- Insulin Semisynthesis.- Ribonuclease Semisynthesis.- Hemoglobin Semisynthesis.- Semisynthetic Protease Inhibitors.- Cytochrome C With Semisynthesis.</p>

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Protein Engineering by Semisynthesis

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